Fig. 5
From: A stable isotope dilution method for a highly accurate analysis of karrikins
Validation of karrikin quantification method using the parameters of recovery (a), matrix effect (b) and process efficiency (c). The recovery (%) was calculated from the peak area of each compound (10 pmol of KAR1 and KAR2) added to the plant extract (5, 10 and 20 mg FW of ten-day-old Arabidopsis seedlings). Matrix effect (%) and process efficiency (%) were calculated with solvent-only calibration in methanol (Cal 1), calibration dissolved in the plant matrix blanks obtained after the SPE step (Cal 2), and matrix-matched calibration prepared similarly to the sample according to developed purification protocol (Cal 3)