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Fig. 3 | Plant Methods

Fig. 3

From: A stable isotope dilution method for a highly accurate analysis of karrikins

Fig. 3

Process efficiency of the SPE-based method for KAR isolation. a, c Recovery (%) of KAR1 and KAR2 purified by four different SPE sorbents (C8, C18, Isolute M-M and Oasis HLB) without (a, 0 mg FW) and with plant matrix (c, 10 mg FW). b Representative test of loading capacity and extraction recoveries at different steps during the purification protocol described in Fig. 4. For all experiments, 10 pmol of each analyte was added to 1 ml of 10% methanol acidified with 0.1% formic acid without and with the presence of a plant tissue. The samples were then extracted and applied on SPE columns. After UHPLC–MS/MS analysis, the KARs' peak areas were compared to the peak’s areas of the original stock and expressed as a percentage recovery. Values are means ± SD (n = 3)

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