TY - JOUR AU - Ziarsolo, Peio AU - Hasing, Tomas AU - Hilario, Rebeca AU - Garcia-Carpintero, Victor AU - Blanca, Jose AU - Bombarely, Aureliano AU - CaƱizares, Joaquin PY - 2021 DA - 2021/03/25 TI - K-seq, an affordable, reliable, and open Klenow NGS-based genotyping technology JO - Plant Methods SP - 30 VL - 17 IS - 1 AB - K-seq, a new genotyping methodology based on the amplification of genomic regions using two steps of Klenow amplification with short oligonucleotides, followed by standard PCR and Illumina sequencing, is presented. The protocol was accompanied by software developed to aid with primer set design. SN - 1746-4811 UR - https://doi.org/10.1186/s13007-021-00733-6 DO - 10.1186/s13007-021-00733-6 ID - Ziarsolo2021 ER -