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Fig. 2 | Plant Methods

Fig. 2

From: Isotope ratio-based quantification of carbon assimilation highlights the role of plastidial isoprenoid precursor availability in photosynthesis

Fig. 2

Workflow for whole plant isotopic labeling and quantification of 13C label by EA-IRMS. Solid arrows signify the order of steps. Hollow arrows represent gas flow. Both air sources (normal and labeled air) were supplied by compressed air tanks containing 400 μL L−1 CO2 or 13CO2 (99% enrichment) in a mixture of nitrogen:oxygen (80:20). Air was (de)humidified by passing through a chilled wash bottle containing water. CO2 and H2O vapor were quantified before entering the cuvette (reference) and in the cuvette exhaust (sample). After processing labeled plant tissue, 2 mg aliquots were analyzed by EA-IRMS, which consisted of combustion of the sample to carbon dioxide, separation in a magnetic sector mass analyzer, and data acquisition

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Plant Methods

ISSN: 1746-4811