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Fig. 3 | Plant Methods

Fig. 3

From: A label-free, fast and high-specificity technique for plant cell wall imaging and composition analysis

Fig. 3

Detection and acquisition of CRS images. a Control software setting. Turn on the key switch and power switch of the laser control unit. Then turn on the power switch of the chiller and check whether the water-cooled pipe joint is leaking. Set the temperature to 23 ℃. Wait about 0.5 h for the temperature to stabilize. b Dialog window for detection channel settings. Switch on the panel PC. The control software (picoEmerald ver 3.0.2.0) will start automatically (a). Confirm by clicking “YES” when the system asks you whether to start the laser. This takes about 0.5 h to warm up and light the laser. c Dialog window for image settings. Turn on the main power switch, key switch, scanning module, and motorized translation stage of the microscope. d Dialog window for SRS detection settings. Open the FV10-ASW3.0 software of the microscope by double-clicking the FV10-ASW3.0 icon. The RDX4 channel is for CARS imaging, while the AL1 channel is for SRS imaging

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Plant Methods

ISSN: 1746-4811