Fig. 6
From: New methods for confocal imaging of infection threads in crop and model legumes
Rhodamine-123-PAS/calcofluor white labelling is more effective than GFP-tagged rhizobia for imaging infection threads in whole roots. Inoculation zones in whole M. truncatula roots at equivalent stages of nodule development. a Optical section through cleared inoculation zone labelled with rhodamine-123 (magenta) and calcofluor white (green) shows separate nodules; all cell layers; and infection threads (white arrows) and an infection chamber (inset black arrow). b An equivalent position in a fresh root inoculated with GFP-tagged rhizobia. Root hairs imaged by DIC are poorly defined and no internal cell layers are visible due to the imaging depth. Inset shows GFP-tagged rhizobia in an infection chamber (arrow). Images are 4 × 6 tile scans captured using a 40× objective. Scale bars equal 500 µm, and 50 µm (inset)