Fig. 5
From: New methods for confocal imaging of infection threads in crop and model legumes
Rhodamine-123-PAS/calcofluor white labelling also differentiates several other tissue types. Stomata thickened inner cell walls (a, arrow); xylem vessels (white arrow) and mature endodermis (black arrow) (b); casparian strips (c, arrow); and non-cellulosic cell wall matrix (d, arrow) in M. truncatula; and trichomes of L. japonicus (e, arrow) are labelled with rhodamine-123 (magenta), in contrast to surrounding cell walls labelled with calcofluor white (green). Images a and c–e are maximum projections of 7.14 µm (a), 3.57 µm (c), 1 µm (d), and 30 µm (e) confocal z-stacks. Images a–d were captured using a 63× objective. Image e was captured using a 40× objective. Scale bar equals 50 µm