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Fig. 4 | Plant Methods

Fig. 4

From: New methods for confocal imaging of infection threads in crop and model legumes

Fig. 4

Rhodamine-123-PAS/calcofluor white labelling of infection threads in four legumes. Root hair and nodule infection threads (arrows), respectively, in M. truncatula (a, b), T. repens (c, d), and L. japonicus (e, g). Cortical and nodule infection threads in C. arietinum (f, h). Rhodamine-123 is shown in magenta; calcofluor white is shown in green. a, c, e Infection threads (arrows) in all but T. repens root hairs (c) are clearly labelled with rhodamine-123. Maximum projections of only the rhodamine-123 channel in much larger z-stacks show extensive infection thread networks in M. truncatula and T. repens nodules (b, d). Images are maximum projections from 5 µm and 6.43 µm (a), 8 µm and 49.97 µm (b), 3.93 µm and 7.14 µm (c), 7.14 µm and 176.46 µm (d), 14.29 µm (e), 37.15 µm (f), 11.43 µm (g), and 20 µm (h) confocal z-stacks. Images a–g were captured using a 63× objective. Image h was captured using a 40× objective. Scale bars equal 25 µm (a, c, e, f) and 50 µm (b, d, g, h). rh root hair, en endodermis, cc cortical cell. Original resolution versions of images b and d are available in Additional file 1: Figures S2, S3

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