Evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques

Table 4 Primer combinations and polymorphism rate

Primer combination	Primer sequence (5′ to 3′)	Total number of bands	Number of polymorphic bands	Polymorphism rate (%)
EAGG-MGT	GACTGCGTACCAATTCAGG GATGAGTCCTGAGTAAGT	37	28	75.67
ECT-MGAG	GACTGCGTACCAATTCCT GATGAGTCCTGAGTAAGAG	45	42	93.33
ECT-MGC	GACTGCGTACCAATTCCT GATGAGTCCTGAGTAAGC	37	32	86.48
EAGC-MCAA	GACTGCGTACCAATTCAGC GATGAGTCCTGAGTAACAA	50	50	100
MGT-ETA	GATGAGTCCTGAGTAAGT GACTGCGTACCAATTCTA	33	27	81.81
MGC-ETA	GATGAGTCCTGAGTAAGC GACTGCGTACCAATTCTA	55	25	45.45
EGA-MCAT	GACTGCGTACCAATTCGA GATGAGTCCTGAGTAACAT	39	34	87.17
MCT-EAGG	GATGAGTCCTGAGTAACT GACTGCGTACCAATTCAGG	28	21	75
MCAG-EAT	GATGAGTCCTGAGTAAGA GACTGCGTACCAATTCAT	71	63	88.73
EAGG- MCAG	GACTGCGTACCAATTCAGG GATGAGTCCTGAGTAAGA	28	25	89.28
EAGG-MTTT	GACTGCGTACCAATTCAGG GATGAGTCCTGAGTAATTT	35	16	45.71
EAGG-MGC	GACTGCGTACCAATTCAGG GATGAGTCCTGAGTAAGC	41	16	39.02
Total/Average		499	379	75.95

ISSN: 1746-4811