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Table 8 Phenotypic analysis of progenies of plants regenerated from Ws-2 protoplasts

From: Optimization of protoplast regeneration in the model plant Arabidopsis thaliana

  Wild-type R1 #1 R1 #2 R1 #3
Seed germination (%) 98.04 ± 0.80 97.06 ± 1.39 100 99.02 ± 0.80
Cotyledon area (mm2) 4.84 ± 0.15 4.88 ± 0.22 5.16 ± 0.22 4.93 ± 0.21
Flowering time (Rosette leaf no.) 8.37 ± 0.75 8.62 ± 0.85 8.27 ± 0.70 8.69 ± 0.67
  1. Harvested R1 seeds from protoplast-regenerated plants were germinated on MS medium (> 60 seeds). Germination rate was calculated as the percentage of radicle emergence from the seed coat at 2 days after transfer to the long-day condition. Cotyledon area of 7-day-old seedlings was measured with ImageJ software. Flowering time was assessed as the number of rosette leaves, when the bolt reached 1 cm. Data represent the mean ± SEM of biological triplicates
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Plant Methods

ISSN: 1746-4811