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Table 5 Shoot regeneration efficiency on three media for de novo shoot organogenesis

From: Optimization of protoplast regeneration in the model plant Arabidopsis thaliana

Ecotypes Media
SIM (%) SRMA (%) SRA (%)
Col-0 18.67 ± 1.09 13.33 ± 2.88 6.67 ± 2.18
Ws-2 96.00 ± 3.27 93.33 ± 3.93 94.67 ± 2.88
No-0 30.67 ± 5.76 14.67 ± 6.62 10.67 ± 1.09
HR-10 76.00 ± 4.99 50.67 ± 3.93 12.00 ± 1.89
  1. CIM-grown microcalli with a diameter of 1–2 mm were transferred to three different media for shoot regeneration. After 3-week incubation, shoot regeneration rate was calculated as the percentage of calli with emerging shoots relative to the total number of calli with a size of > 1 mm in a diameter. Data represent the mean ± SEM of biological triplicates