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Table 5 Shoot regeneration efficiency on three media for de novo shoot organogenesis

From: Optimization of protoplast regeneration in the model plant Arabidopsis thaliana

Ecotypes

Media

SIM (%)

SRMA (%)

SRA (%)

Col-0

18.67 ± 1.09

13.33 ± 2.88

6.67 ± 2.18

Ws-2

96.00 ± 3.27

93.33 ± 3.93

94.67 ± 2.88

No-0

30.67 ± 5.76

14.67 ± 6.62

10.67 ± 1.09

HR-10

76.00 ± 4.99

50.67 ± 3.93

12.00 ± 1.89

  1. CIM-grown microcalli with a diameter of 1–2 mm were transferred to three different media for shoot regeneration. After 3-week incubation, shoot regeneration rate was calculated as the percentage of calli with emerging shoots relative to the total number of calli with a size of > 1 mm in a diameter. Data represent the mean ± SEM of biological triplicates