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Table 3 Efficiency of callus formation from Ca2+-alginate hydrogel-embedded protoplasts

From: Optimization of protoplast regeneration in the model plant Arabidopsis thaliana

Ecotypes Media
CIM (Calli cm−2) CMC (Calli cm−2) PCA (Calli cm−2)
Col-0 5.75 ± 0.62 4.96 ± 0.35 6.34 ± 0.44
Ws-2 6.63 ± 0.40 4.88 ± 0.29 3.09 ± 0.57
No-0 6.11 ± 0.43 4.45 ± 0.46 6.43 ± 0.35
HR-10 6.37 ± 0.16 5.42 ± 0.30 4.04 ± 0.10
  1. The hydrogels were incubated in PIM, followed by a 2-week incubation in three different media for callus proliferation. The number of dividing calli with a diameter of > 1 mm in a fixed area of hydrogel area (21.50 cm2) was measured. Data represent the mean ± SEM of biological triplicates