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Table 3 Efficiency of callus formation from Ca2+-alginate hydrogel-embedded protoplasts

From: Optimization of protoplast regeneration in the model plant Arabidopsis thaliana

Ecotypes

Media

CIM (Calli cm−2)

CMC (Calli cm−2)

PCA (Calli cm−2)

Col-0

5.75 ± 0.62

4.96 ± 0.35

6.34 ± 0.44

Ws-2

6.63 ± 0.40

4.88 ± 0.29

3.09 ± 0.57

No-0

6.11 ± 0.43

4.45 ± 0.46

6.43 ± 0.35

HR-10

6.37 ± 0.16

5.42 ± 0.30

4.04 ± 0.10

  1. The hydrogels were incubated in PIM, followed by a 2-week incubation in three different media for callus proliferation. The number of dividing calli with a diameter of > 1 mm in a fixed area of hydrogel area (21.50 cm2) was measured. Data represent the mean ± SEM of biological triplicates
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Plant Methods

ISSN: 1746-4811