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Table 2 Percentage of dividing protoplasts of four different Arabidopsis ecotypes in three different media

From: Optimization of protoplast regeneration in the model plant Arabidopsis thaliana

Ecotypes

Media

PIM (protoplasts mm−2)

CMA (protoplasts mm−2)

PCA (protoplasts mm−2)

Col-0

14.83 ± 3.84

14.71 ± 2.06

2.53 ± 0.96

Ws-2

16.32 ± 2.21

13.10 ± 3.48

2.18 ± 0.85

No-0

13.68 ± 3.34

10.92 ± 2.63

1.49 ± 0.87

HR-10

15.75 ± 3.56

1.84 ± 1.49

5.86 ± 1.49

  1. Protoplasts were embedded in Ca2+-alginate hydrogels, which were incubated in three different media for 2 weeks. The number of dividing protoplasts in a unit area of hydrogel was calculated. Data represent the mean ± SEM of biological triplicates