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Table 2 Mutation efficiency of AtBRI1 gene in system leaf and transgenic M1 generation

From: Heritable gene editing using FT mobile guide RNAs and DNA viruses

Transgenic receptor Modification Target System leaf mutation frequency of M0 Plant number No. of M1 edited plants showing excepted phenotype No. of M1 edited plants Mutation frequency of M1
Pro35S::Cas9 Unmodified sgRNA BRI1-sgRNA (3/12) 25% #2 N/A 0 0.00%(0/68)
#4 N/A 0 0.00%(0/48)
#7 N/A 0 0.00%(0/72)
FT-sgRNA FT-BRI1-sgRNA (3/12) 25% #2 N/A 3 4.35%(3/69)
#6 N/A 4 8.33%(4/48)
#10 N/A 8 8.79%(8/91)
ProYao::Cas9 Unmodified sgRNA BRI1-sgRNA (8/16)50% #12 N/A 0 0.00%(0/44)
#13 N/A 0 0.00%(0/60)
#14 N/A 0 0.00%(0/64)
FT-sgRNA FT-BRI1-sgRNA (3/12) 25% #3 N/A 3 6.52%(3/46)
#4 N/A 5 8.06%(5/62)
#11 N/A 3 5.56%(3/54)