Table 2 Mutation efficiency of AtBRI1 gene in system leaf and transgenic M1 generation
From: Heritable gene editing using FT mobile guide RNAs and DNA viruses
Transgenic receptor | Modification | Target | System leaf mutation frequency of M0 | Plant number | No. of M1 edited plants showing excepted phenotype | No. of M1 edited plants | Mutation frequency of M1 |
|---|---|---|---|---|---|---|---|
Pro35S::Cas9 | Unmodified sgRNA | BRI1-sgRNA | (3/12) 25% | #2 | N/A | 0 | 0.00%(0/68) |
#4 | N/A | 0 | 0.00%(0/48) | ||||
#7 | N/A | 0 | 0.00%(0/72) | ||||
FT-sgRNA | FT-BRI1-sgRNA | (3/12) 25% | #2 | N/A | 3 | 4.35%(3/69) | |
#6 | N/A | 4 | 8.33%(4/48) | ||||
#10 | N/A | 8 | 8.79%(8/91) | ||||
ProYao::Cas9 | Unmodified sgRNA | BRI1-sgRNA | (8/16)50% | #12 | N/A | 0 | 0.00%(0/44) |
#13 | N/A | 0 | 0.00%(0/60) | ||||
#14 | N/A | 0 | 0.00%(0/64) | ||||
FT-sgRNA | FT-BRI1-sgRNA | (3/12) 25% | #3 | N/A | 3 | 6.52%(3/46) | |
#4 | N/A | 5 | 8.06%(5/62) | ||||
#11 | N/A | 3 | 5.56%(3/54) |