TY - JOUR AU - Sasani, Nadia AU - Bock, Peter AU - Felhofer, Martin AU - Gierlinger, Notburga PY - 2021 DA - 2021/02/08 TI - Raman imaging reveals in-situ microchemistry of cuticle and epidermis of spruce needles JO - Plant Methods SP - 17 VL - 17 IS - 1 AB - The cuticle is a protective layer playing an important role in plant defense against biotic and abiotic stresses. So far cuticle structure and chemistry was mainly studied by electron microscopy and chemical extraction. Thus, analysing composition involved sample destruction and the link between chemistry and microstructure remained unclear. In the last decade, Raman imaging showed high potential to link plant anatomical structure with microchemistry and to give insights into orientation of molecules. In this study, we use Raman imaging and polarization experiments to study the native cuticle and epidermal layer of needles of Norway spruce, one of the economically most important trees in Europe. The acquired hyperspectral dataset is the basis to image the chemical heterogeneity using univariate (band integration) as well as multivariate data analysis (cluster analysis and non-negative matrix factorization). SN - 1746-4811 UR - https://doi.org/10.1186/s13007-021-00717-6 DO - 10.1186/s13007-021-00717-6 ID - Sasani2021 ER -