TY - JOUR AU - de Almeida, Natália Pinto AU - Neto, Domingos Ferreira Mélo AU - Carneiro, Gabriel Reis Alves AU - de Farias, Andreza Raquel Barbosa AU - Domont, Gilberto Barbosa AU - de Paiva Campos, Francisco de Assis AU - Nogueira, Fábio César Sousa PY - 2021 DA - 2021/02/06 TI - Monitoring casbene synthase in Jatropha curcas tissues using targeted proteomics JO - Plant Methods SP - 15 VL - 17 IS - 1 AB - Casbene synthase (CS) is responsible for the first committed step in the biosynthesis of phorbol esters (PE) in the Euphorbiaceae. PE are abundant in the seeds of the biofuel crop Jatropha curcas and its toxicity precludes the use of the protein-rich cake obtained after oil extraction as an animal feed and the toxicity of the fumes derived from burning PE containing biofuel is also a matter of concern. This toxicity is a major hindrance to exploit the potential of this crop as a source of raw material to produce biodiesel. For this reason, the current research on J. curcas is mainly focused on the understanding of the biosynthesis and site of synthesis of PE, as an avenue for the development of genotypes unable to synthesize PE in its seeds. SN - 1746-4811 UR - https://doi.org/10.1186/s13007-021-00716-7 DO - 10.1186/s13007-021-00716-7 ID - de Almeida2021 ER -