Fig. 3

Lolium perenne monocrop column experiment NO₃⁻-selective sensor data. Mean data (4 columns) are shown for each treatment as indicated in Table 1 as the thickest coloured lines. NO₃⁻-selective sensor data are shown for top (yellow), middle (orange), and bottom (brown) levels of the columns. Data is the 12 h average of four experimental replicates in GraphPad Prism 7 (GraphPad Software Inc.), with standard error of the mean indicated with thinner lines of the same colour. Overlayed coloured vertical bars indicate the treatment for the L. perenne crop planted (green), nitrate application at day 0 (blue), or defoliation of total aboveground vegetative biomass at day 28 (purple). Note no nitrate was added to Monocrop 1 columns