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Fig. 3 | Plant Methods

Fig. 3

From: Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton

Fig. 3

Graphic drawing of the workflow of barcoding. a Schematic represent generated plants used for barcoding. b The genomic DNA of every generated plants was extracted individually. c DNA used for PCR-based barcodes library construction went through one round of amplification and each sample was labeled independently with unique barcodes. d DNA library of all tested plants. DNA library was built by pooling the positive PCR mixture together as one sample in equal amount and were adjusted to NGS. Positive PCR results indicated the presence of the T-DNA insertion harboring the sgRNA. e High throughput sequencing for the DNA library. f Statistics of high throughput sequencing results identified number of sgRNAs existed in each plant

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Plant Methods

ISSN: 1746-4811