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Fig. 2 | Plant Methods

Fig. 2

From: Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton

Fig. 2

Different stages of tissue culture during the generation of CRISPR-Cas9 pooled sgRNA assembly mutants by Agrobacterium-mediated transformation in cotton. a Transformed hypocotyls were placed on co-culture medium after treated with Agrobacterium suspension. b Transformed hypocotyls on 2,4 D medium containing kanamycin and cephalosporin antibiotics. c Hypocotyls after 4 months of transformation on 2,4D medium. d Two months old differentiated calli on differentiation medium. e Plantlets were transferred into rooting medium. f Transgenic plants into the Hoagland medium for more adaptation. g Transgenic plants were transferred into small pots. h, i Transgenic plants were grown into big pots in the greenhouse

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Plant Methods

ISSN: 1746-4811