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Fig. 4 | Plant Methods

Fig. 4

From: A cucumber green mottle mosaic virus vector for virus-induced gene silencing in cucurbit plants

Fig. 4

Silencing efficiency of VIGS vectors carrying PDS fragments of different sizes on cucurbits. a Indicate the uninoculated leaves displaying most obvious photobleaching on watermelon plants at 32 dpi, on melon at 27 dpi, on cucumber at 39 dpi and on bottle gourd at 34 dpi, respectively. Bar = 2, 3, 3 and 3 cm (from top to bottom). b Real-time qRT-PCR analysis of PDS expression in noninfected (NI), pV190 empty vector (EV), and CGMMV-PDS-infected cucurbit (watermelon, melon, cucumber and bottle gourd) plants. Three technical replicates were performed for each individual sample (*P < 0.05 and **P < 0.01 ***P < 0.001 compared with the empty vector (pV190) by Student’s t test. Error bars indicate the SD

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