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Fig. 2 | Plant Methods

Fig. 2

From: Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography

Fig. 2

Contrast enhancement. Corresponding CT slices from three different treatments are displayed. A dry seed was directly mounted on the tip of a metal rod (φ 3 mm × 55 mm) with a double-sided tape and the rod was set on the sample stage of the nano3DX-sCMOS with the Cu-target. a Low resolution observation. For the data collection, 1000 frames with a 1.44 s exposure per frame were taken by the continuous-scan mode with a voxel size of (1.27 μm)3 (L1080 lens, bin 1, sample-to-detector distance of 4 mm). b High resolution observation. For the data collection, 300 frames with a 2.40 s exposure per frame were taken by the continuous-scan mode with a voxel size of (0.63 μm)3 (L0270 lens, bin 2, sample-to-detector distance of 4 mm). Scale bars: 100 μm in a and 20 μm in b

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Plant Methods

ISSN: 1746-4811