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Fig. 3 | Plant Methods

Fig. 3

From: Can-Seq: a PCR and DNA sequencing strategy for identifying new alleles of known and candidate genes

Fig. 3

Missense RDR6 mutation in EMS#159 (R828K), but not in EMS#146 (P1073L) or EMS#157 (G19E), is a putative new rdr6 allele. a Rosette phenotypes of EMS#153 (W227*), EMS#157 (G19E), EMS#146 (P1073L), EMS#159 (R828K) and 10027-3 wild type (WT). The 10027-3 wild type shows systemic post-transcriptional gene silencing (PTGS) of GFP. Based on backcrosses to the 10027-3 wild type and analysis of the BC1F1 phenotype and BC1F2 segregation, the rtp phenotypes of EMS#153, EMS#157, EMS#146 and EMS#159 are inherited as recessive traits. bc EMS#153 was complemented by EMS#157 and EMS#146, and F1 plants from these crosses showed almost complete systemic PTGS of GFP. d EMS#153 was not complemented by EMS#159 and F1 plants from this cross showed defective systemic RNAi of GFP. e Location of the new and putative rdr6 alleles recovered by Can-Seq in the RDR6 locus (AT3G49500). Exon and intron sequences are indicated by thick and narrow lines, respectively. Rosette images are of plants grown in soil under long days for four weeks after planting

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