A high-throughput screening method to identify proteins involved in unfolded protein response of the endoplasmic reticulum in plants

Table 1 List of primers used for promoter and gene isolation, and relative gene expression measurement by qRT-PCR

Purpose/name	Primer sequence		References
Promoters
pNtbZIP60	F	aacaggtctcaacctGGTAAGGTTGCCGTAGTAAAAG	N/A
pNtbZIP60	R	aacaggtctcatgttCGCCTATTCTACAACCCAGA	N/A
pNtSKP1	F	aacaggtctcaacctCGACACGTTTGGTAGACTCATC	N/A
pNtSKP1	R	aacaggtctcatgttCGTAGCAACACTAACCCTAG	N/A
pAtBIP1	F	aacaggtctcaacctAGAGGAGGTTGAGAGAGAAGATAGAC	N/A
pAtBIP1	R	aacaggtctcatgttATCGGAAACTTTTGCGTACGAT	N/A
pAtBIP3	F	aacaggtctcaacctTGCATCGGGAAATCTTGTTT	N/A
pAtBIP3	R	aacaggtctcatgttTTTTCGTTGTTGAGAACTCTTCTT	N/A
Genes
eYFP	F	atatggtctcaggctctATGGTGAGCAAGGGCGAGGA	N/A
eYFP	R	atatggtctcactgaCTTGTACAGCTCGTCCATGCCGAGAG	N/A
AtIRE1a	F	atatggtctcaggctccATGCCGCCGAGATGTCCT	N/A
AtIRE1a	R	atatggtctcactgaTTAGATGATGTCGCATTTGAAGTACTTTC	N/A
AtHY5	F	atatggtctcaggctccATGCAGGAACAAGCGACTAGC	N/A
AtHY5	R	atatggtctcactgaTCAAAGGCTTGCATCAGCATT	N/A
qRT-PCR
Nt18S	F	ATGGCCGTTCTTAGTTGGTGGAGC	Ye et al. [51]
Nt18S	R	AGTTAGCAGGCTGAGGTCTCGAAC	Ye et al. [51]
NtbZIP60	F	CCTGCTTTGGTTCATGGGCATCAT	Ye et al. [51]
NtbZIP60	R	AGAAGACCGTGGTTTCTGCTTCGT	Ye et al. [51]
NbCNX1	F	ATCTTTGGCGGGAAGAAGC	N/A
NbCNX1	R	TCCTCTGTAGCTCCTTGGCTGT	N/A
NbSKP1	F	GGCTGCCAACTATTTGAACA	Shen et al. [43]
NbSKP1	R	CATTCTCCCTCCTGACTTCTT	Shen et al. [43]
NtPR1F	F	CCGTTGAGATGTGGGTCAAT	Hamorsky et al. [11]
NtPR1F	R	CGCCAAACCACCTGAGTATAG	Hamorsky et al. [11]

Small letters in the primer sequence represent adapters for golden gate cloning, compatible with the GreenGate vector set [23]. F and R represent forward and reverse primer sequences, respectively

ISSN: 1746-4811