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Table 1 List of primers used for promoter and gene isolation, and relative gene expression measurement by qRT-PCR

From: A high-throughput screening method to identify proteins involved in unfolded protein response of the endoplasmic reticulum in plants

Purpose/namePrimer sequenceReferences
Promoters
 pNtbZIP60FaacaggtctcaacctGGTAAGGTTGCCGTAGTAAAAGN/A
RaacaggtctcatgttCGCCTATTCTACAACCCAGA
 pNtSKP1FaacaggtctcaacctCGACACGTTTGGTAGACTCATCN/A
RaacaggtctcatgttCGTAGCAACACTAACCCTAG
 pAtBIP1FaacaggtctcaacctAGAGGAGGTTGAGAGAGAAGATAGACN/A
RaacaggtctcatgttATCGGAAACTTTTGCGTACGAT
 pAtBIP3FaacaggtctcaacctTGCATCGGGAAATCTTGTTTN/A
RaacaggtctcatgttTTTTCGTTGTTGAGAACTCTTCTT
Genes
 eYFPFatatggtctcaggctctATGGTGAGCAAGGGCGAGGAN/A
RatatggtctcactgaCTTGTACAGCTCGTCCATGCCGAGAG
 AtIRE1aFatatggtctcaggctccATGCCGCCGAGATGTCCTN/A
RatatggtctcactgaTTAGATGATGTCGCATTTGAAGTACTTTC
 AtHY5FatatggtctcaggctccATGCAGGAACAAGCGACTAGCN/A
RatatggtctcactgaTCAAAGGCTTGCATCAGCATT
qRT-PCR
 Nt18SFATGGCCGTTCTTAGTTGGTGGAGCYe et al. [51]
RAGTTAGCAGGCTGAGGTCTCGAAC
 NtbZIP60FCCTGCTTTGGTTCATGGGCATCATYe et al. [51]
RAGAAGACCGTGGTTTCTGCTTCGT
 NbCNX1FATCTTTGGCGGGAAGAAGCN/A
RTCCTCTGTAGCTCCTTGGCTGT
 NbSKP1FGGCTGCCAACTATTTGAACAShen et al. [43]
RCATTCTCCCTCCTGACTTCTT
 NtPR1FFCCGTTGAGATGTGGGTCAATHamorsky et al. [11]
RCGCCAAACCACCTGAGTATAG
  1. Small letters in the primer sequence represent adapters for golden gate cloning, compatible with the GreenGate vector set [23]. F and R represent forward and reverse primer sequences, respectively