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Fig. 5 | Plant Methods

Fig. 5

From: A high-throughput screening method to identify proteins involved in unfolded protein response of the endoplasmic reticulum in plants

Fig. 5

Reproducibility, sources of variability, and confirmation of a protein that interferes with unfolded protein response (UPR) signaling. Variability in enhanced yellow fluorescent protein (eYFP) fluorescence from 2 independent replicates in both a DMSO (mock) and b tunicamycin (Tm) treated leaves. c Estimated variation between replicates of protein expression based on mCherry (mCh) fluorescence in Tm infiltrated samples. d Relative gene expression of UPR marker genes in samples expressing UMAG_0592724-370. e eYFP and f mCh fluorescence decrease as a function of sample number. Error bars represent standard deviation, asterisks represent statistically significant differences (one-way ANOVA or t-test) between samples: *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001, when statistical analysis was performed. n.s. not significant, a.u. arbitrary units

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Plant Methods

ISSN: 1746-4811