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Fig. 4 | Plant Methods

Fig. 4

From: A high-throughput screening method to identify proteins involved in unfolded protein response of the endoplasmic reticulum in plants

Fig. 4

Pilot screen to identify proteins that influence unfolded protein response (UPR) signaling using a subset of an effector library from the biotrophic plant pathogen U. maydis. enhanced yellow fluorescent protein (eYFP) fluorescence in a DMSO (mock) treated samples to identify proteins that induce UPR signaling, and in b samples where ER stress was induced (Tm) to identify proteins that inhibit UPR signaling. In both cases, samples of plants expressing mCherry (mCh) were used as a reference. Grey lines represent the average fluorescence (full line) and standard deviation (dashed lines) in mCh samples. Error bars represent standard deviation, asterisks represent statistically significant differences (one-way ANOVA or t-test) between samples: *P ≤ 0.01, **P ≤ 0.001. a.u. arbitrary units

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Plant Methods

ISSN: 1746-4811