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Table 1 Summary of the advantages and disadvantages of different methods for the establishment of a split-root system available in the literature

From: Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level

Method

Source

Destructive procedure required?

Technically challenging?

Achievable in young seedlings?

Applicable in Arabidopsis?

Schematic representation of the technique

Splitting of the developed root system

[9]

No

No

No

Yes

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Splitting of newly forming lateral roots

[5]

No

No

Yes

No

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Cutting longitudinally and Splitting the main root

[6]

Yes

Yes

Yes

No*

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Inverted Y-grafting

[11]

Yes**

Yes

Yes

Yes

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Cutting the whole root, splitting the new roots

[8]

Yes

No

Yes

Yes

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  1. * Unless the researcher possesses surgeon-like skills
  2. ** Even though no part of the plant is removed, it requires making an incision on the root in order to insert the graft