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Fig. 6 | Plant Methods

Fig. 6

From: Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level

Fig. 6

Visualization of the cytokinin signalling in Arabidopsis ARR5::GUS transgenic lines (a). 10 µM tZ were applied to one side of the SRS (+ Root) and the increase on expression of ARR5 was observed on the other half (−Root). Picture of Arabidopsis DEX-inducible CKX overexpressing plants transplanted into the custom SRS dishes (b) containing patches of MS () and MS + DEX (+) at the moment of transplant (left) and after 5 days (right). RT-qPCR analysis of the relative expression of HvCKX and ARR4, 5 and 6 on roots of Arabidopsis proCaMV35S > GR > HvCKX2 line (CKX-c) after either the whole root system (CKX DEX) or half of the SRS had been activated (CKX SR; + is the activated half and − the non-activated one). Asterisks indicated statistically significant differences respect to non-activated CKX plants (DMSO) for p < 0.05

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