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Table 2 Effect of blank-correction methods on the test well absorbance values in Fig. 9

From: Screening natural product extracts for potential enzyme inhibitors: protocols, and the standardisation of the usage of blanks in α-amylase, α-glucosidase and lipase assays

Method

Formula

Calculation

Blank-corrected absorbance

1

RD − BB

0.750 − 0.050

0.700

2

RD − SuB

0.750 − 0.150

0.600

3

RD − SaB

0.750 − 0.250

0.500

4

RD − (BB + SaB)

0.750 − (0.050 + 0.250)

0.450

5

RD − (SuB + SaB)

0.750 − (0.150 + 0.250)

0.350

6

RD − [(SuB − BB) + SaB)]

0.750 − [(0.150 − 0.050) + 0.250]

0.400

  1. RD: raw data (Atest); BB: buffer blank; SuB: substrate blank, SaB: sample blank