Method | Formula for blank-correction | |
---|---|---|
α-glucosidase assay | α-amylase and lipase assays | |
1 | \({\text{A}}_{\text{test}} - {\text{A}}_{\text{buffer blank}}\) | \({\text{F}}_{\text{test}} - {\text{F}}_{\text{buffer blank}}\) |
2 | \({\text{A}}_{\text{test}} - {\text{A}}_{\text{substrate blank}}\) | \({\text{F}}_{\text{test}} - {\text{F}}_{\text{substrate blank}}\) |
3 | \({\text{A}}_{\text{test}} - {\text{A}}_{\text{sample blank}}\) | \({\text{F}}_{\text{test}} - {\text{F}}_{\text{sample blank}}\) |
4 | \({\text{A}}_{\text{test}} - ( {\text{A}}_{\text{buffer blank}} {\text{ + A}}_{\text{sample blank}} )\) | \({\text{F}}_{\text{test}} - ( {\text{F}}_{\text{buffer blank}} {\text{ + F}}_{\text{sample blank}} )\) |
5 | \({\text{A}}_{\text{test}} - ( {\text{A}}_{\text{substrate blank}} {\text{ + A}}_{\text{sample blank}} )\) | \({\text{F}}_{\text{test}} - ( {\text{F}}_{\text{substrate blank}} {\text{ + F}}_{\text{sample blank}} )\) |
6 | \({\text{A}}_{\text{test}} - [\left( {{\text{A}}_{\text{substrate blank}} - {\text{A}}_{\text{buffer blank}} } \right){\text{ + A}}_{\text{sample blank}} ]\) | \({\text{F}}_{\text{test}} - [\left( {{\text{F}}_{\text{substrate blank}} - {\text{F}}_{\text{buffer blank}} } \right){\text{ + F}}_{\text{sample blank}} ]\) |