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Fig. 8 | Plant Methods

Fig. 8

From: Screening natural product extracts for potential enzyme inhibitors: protocols, and the standardisation of the usage of blanks in α-amylase, α-glucosidase and lipase assays

Fig. 8

Effect of orlistat and plant extracts on porcine pancreatic lipase activity. Orlistat (positive control), and the Camellia sinensis and Sophora japonica extracts were tested at 0.1 mg/mL. The negative control (uninhibited control) was normalised to 100% activity. Data represent the mean + SEM of readings from three independent experiments. Bars that do not share a letter were significantly different from each other as determined by one-way ANOVA and Tukey’s multiple comparison test (p < 0.05). Uppercase letters denote significance between treatment groups and lowercase letters denotes significance within groups. RD: raw data, BB: buffer blank (contained buffer only), SuB: substrate blank (contained substrate + buffer), and SaB: sample blank. Sample blank composition: C. sinensis sample blank = C. sinensis extract + buffer, S. japonica sample blank = S. japonica extract + buffer, orlistat sample blank (positive control blank) = orlistat + buffer, and control sample blank (negative control blank) = vehicle + buffer (as the vehicle was buffer, this contained buffer only)

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Plant Methods

ISSN: 1746-4811