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Fig. 7 | Plant Methods

Fig. 7

From: Screening natural product extracts for potential enzyme inhibitors: protocols, and the standardisation of the usage of blanks in α-amylase, α-glucosidase and lipase assays

Fig. 7

Effect of acarbose and plant extracts on porcine pancreatic α-amylase activity. Acarbose (positive control), and the Gardenia jasminoides and Nelumbo nucifera extracts were tested at 0.3 mg/mL. The negative control (uninhibited control) was normalised to 100% activity. Data represent the mean + SEM of readings from three independent experiments. Bars that do not share a letter were significantly different from each other as determined by one-way ANOVA and Tukey’s multiple comparison test (p < 0.05). Uppercase letters denotes significance between treatment groups and lowercase letters denote significance within groups. RD: raw data, BB: buffer blank (contained buffer only), SuB: substrate blank (contained substrate + buffer), and SaB: sample blank. Sample blank composition: G. jasminoides sample blank = G. jasminoides extract + buffer, N. nucifera sample blank = N. nucifera extract + buffer, acarbose sample blank (positive control blank) = acarbose + buffer, and control sample blank (negative control blank) = vehicle + buffer (as the vehicle was buffer, this contained buffer only)

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Plant Methods

ISSN: 1746-4811