Fig. 4
The phenotypes of transgenic plants overexpressing the truncated EMB2654-11M are dependent on RDR6. a Overexpression of the truncated EMB2654-11M in WT background leads to chlorosis phenotype (bar = 1.5 cm). b RT-PCR analysis of the endogenous and total EMB2654 transcripts level in the cosuppression lines. c RT-qPCR analysis of the endogenous EMB2654 transcripts level in the cosuppression lines. d RT-qPCR analysis of the total EMB2654 transcripts level in the cosuppression lines. e RT-qPCR analysis of the splicing efficiency of plastid rps12 intron1 in the cosuppression lines. f Phenotype of transgenic plants overexpressing EMB2654-11M in rdr6-11 (bar = 1.5 cm). g RT-PCR analysis of the endogenous and total EMB2654 transcripts level in the transformants. h RT-qPCR analysis of the endogenous EMB2654 transcripts level in the transformants. i RT-qPCR analysis of the total EMB2654 transcript level in transgenic lines. j Splicing efficiency of plastid rps12 intron1 in the transformants. For RT-qPCR, the values are means of three technique replicates (bars indicate SD). Asterisks indicate significant differences between wild type (WT) and transgenic plants (Student’s t test, *, P < 0.05 and **, P < 0.01). Two to three T1 transformants were analyzed for RT-PCR and qRT-PCR