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Fig. 2 | Plant Methods

Fig. 2

From: A new method for functional analysis of plastid EMBRYO-DEFECTIVE PPR genes by efficiently constructing cosuppression lines in Arabidopsis

Fig. 2

The phenotypes of transgenic plants overexpressing the mutated SOT5 CDS in WT background. a Chlorosis leaf phenotype of transgenic plants overexpressing SOT5-m1 and SOT5-m2 constructs in WT background (bar = 1.5 cm). b RT-PCR analysis of expression levels of the endogenous SOT5, total SOT5 (including endogenous SOT5 and transgene), and two plastid genes in the typical cosuppression lines. c RT-qPCR analysis of the endogenous SOT5 transcript level in the cosuppression lines. d RT-qPCR analysis of the total SOT5 transcript level in the cosuppression lines. e Splicing efficiency of plastid rpl2 and atpF (as a control) in the cosuppression lines. For RT-qPCR, the values are means of three technique replicates (bars indicate SD). Asterisks indicate significant differences between wild type (WT) and transgenic plants (Student’s t test, *, P < 0.05 and **, P < 0.01). Two to three T1 transformants were analyzed for RT-PCR and RT-qPCR

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Plant Methods

ISSN: 1746-4811