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Fig. 1 | Plant Methods

Fig. 1

From: A new method for functional analysis of plastid EMBRYO-DEFECTIVE PPR genes by efficiently constructing cosuppression lines in Arabidopsis

Fig. 1

Schematic diagram of the EMB PPR CDS constructs made in this study and their coding proteins. a Schematic diagram of the EMB PPR CDS constructs. The EMB PPR CDS is indicated by black box. The UTR regions are indicated by black lines. The black arrows show the position of the primer pair to detect the total genes (including endogenous and transgenes). The red arrows show the position of the primer pair to detect the endogenous genes. Bar = 300 bp. b Schematic diagram of the corresponding EMB PPR proteins. The protein structures were predicted by PROSITE (https://prosite.expasy.org/). Pentagons mean PPR motifs in each construct. The amino acid (aa) numbers indicate the size of WT or truncated PPR proteins. Bar = 100 aa

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Plant Methods

ISSN: 1746-4811