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Fig. 5 | Plant Methods

Fig. 5

From: Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato

Fig. 5

TCSv2 responds primarily to CK. a Quantification of TCS driven VENUS expression in wild type Tomato shoot apexes following treatment with indicated hormones. VENUS expression was quantified as arbitrary fluorescent units (AFU) using ImageJ software [62], using images captured with a Nikon stereomicroscope (5–8 shoots per sample). Representative images are shown in Additional file 1: Figure S3. b GUS relative expression was assayed 2 h after treatment with indicated hormones in arabidopsis. Each point represents at least 3 biological replicas ± SE. In both a and b, Student’s t-test (two-tailed) was used for comparison of means, which were deemed significantly different at P ≤ 0.05

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