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Fig. 5 | Plant Methods

Fig. 5

From: Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

Fig. 5

Transfection efficiency in S and M protoplasts isolated from rice calli. The percentage of GFP fluorescence-positive protoplasts were calculated after transfection with pAct1IsGFP-1 to determine plasmid DNA transfection efficiency in S and M callus-derived rice protoplasts of different ages. The transfections were performed in duplicate, with each data point shown. The bar indicates the mean

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Plant Methods

ISSN: 1746-4811