TY - JOUR AU - Poddar, Snigdha AU - Tanaka, Jaclyn AU - Cate, Jamie H. D. AU - Staskawicz, Brian AU - Cho, Myeong-Je PY - 2020 DA - 2020/11/12 TI - Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays JO - Plant Methods SP - 151 VL - 16 IS - 1 AB - An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants. Although rice protoplasts can be isolated from germinated seedlings or cell suspension culture, preparation of those donor tissues can be inefficient, time-consuming, and laborious. Additionally, the lengthy process of protoplast isolation and transfection needs to be completed in a single day. SN - 1746-4811 UR - https://doi.org/10.1186/s13007-020-00692-4 DO - 10.1186/s13007-020-00692-4 ID - Poddar2020 ER -