Fig. 2

Determination of mitochondrial purity. a The existence of the nuclear genome, chloroplast genome and the mitochondrial genome was examined by actin, rubisco and cox III, respectively. M, DNA 2000 marker. b Four components of cells were collected for this experiment. The total lane indicates the protoplasts, the pellet lane indicates lysates from S9 (as outlined in the Sect. “Materials and methods”), the mito lane indicates the mitochondria and the supernatant lane indicates the cytoplasmic, which was derived from the supernatant in S10 (as outlined in the Sect. “Materials and methods”). Rubisco, histone, VDAC and catalase were examined as the marker proteins of the chloroplast, nucleus, mitochondria and peroxisome, respectively