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Fig. 5 | Plant Methods

Fig. 5

From: Rapid metabolite response in leaf blade and petiole as a marker for shade avoidance syndrome

Fig. 5

Time-course experiment of SAS using Raman spectroscopy in leafy vegetables. a Phenotype of leafy vegetables under time-course shade treatment. Basic strategy for shade treatment is the same as shown in Fig. 3a. 3- to 14-days-old Kai Lan and Choy Sum plants were grown in WL, followed by shade treatment in DS for 4 h to 14 days. All plants were of same age during measurement of phenotype and Raman spectroscopy. Scale, 5 cm. b Ratio of petiole length to leaf blade area of plants in a (n = 3). c Carotenoid Raman peaks of time-course shade treatment plants in a (n = 4). Bars and graph denote average ± SE. Statistical significance between control and different durations of shade treatment was determined by two-tailed Student’s t-test: *P < 0.05; **P < 0.01; ***P < 0.001

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