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Fig. 3 | Plant Methods

Fig. 3

From: Rapid metabolite response in leaf blade and petiole as a marker for shade avoidance syndrome

Fig. 3

Raman spectra analysis of WT Arabidopsis and phytochrome mutants, and of WT Arabidopsis at high density planting. a Phenotype of phyB-9BC and phyA-211 after 7 days shade treatment and respective ratios of petiole length to leaf blade area. b Carotenoid Raman peak of plants in a (leaf blade: n = 8, petiole: n = 4). c Total carotenoid content (µg/g fresh weight) of plants in a (n = 3). d Phenotype of 24-days-old WT Arabidopsis plants grown in low to high plant density. Numbers represent plants per pot. Upper panel, top-down view of individual plant  from each pot. Lower panel, side view of  individual plant from each pot. Scale, 5 cm. e Carotenoid Raman peak of Arabidopsis under low to high plant density in d (leaf blade: n = 5, petiole: n = 3). Bars denote average ± SE. WL, white light; MS, moderate shade; DS, deep shade. Statistical significance between control (WL or 1 plant/pot) and the respective treatment (shade or high plant density) was determined by two-tailed Student’s t-test: *P < 0.05; **P < 0.01; ***P < 0.001

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