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Fig. 2 | Plant Methods

Fig. 2

From: Rapid metabolite response in leaf blade and petiole as a marker for shade avoidance syndrome

Fig. 2

Time-course experiment of SAS using Raman spectroscopy in Arabidopsis. a Schematic diagram of different durations of shade treatment. WL, white light; DS, deep shade. b Phenotype of plants under time-course shade treatment. 10-days-old Arabidopsis plants were subjected to a time-course 7 days shade treatment as in a. Upper panel, top-down view of plant. Lower panel, side view of plant. Scale, 1 cm. c Ratio of petiole length to leaf blade area in time-course plants (n = 3). d Carotenoid Raman peak of time-course shade treatment in Arabidopsis plants in b (leaf blade: n = 10, petiole: n = 8). Bars and graph denote average ± SE. Statistical significance between control and different durations of shade treatment was determined by two-tailed Student’s t-test: *P < 0.05; **P < 0.01; ***P < 0.001

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