Table 1 Generation of transgenic plants of hybrid passion fruit KPF4
Transformation experiment | No. of explants infected with Agrobacterium harboring pCAMBIA1301 | No. of explants surviving on selection medium | No. of explants forming shoot buds on SIM with selection agent | No. of shoots developed on SDM with selection agent | Rooting on RIM with selection agent | No. of transgenic lines with GUS expression | No of PCR-positive plant lines | Transformation efficiency (%) |
|---|---|---|---|---|---|---|---|---|
1 | 300 | 18 | 5 | 3 | 2 (66.67%) | 2 | 2 | 0.67 |
2 | 300 | 29 | 6 | 4 | 1 (25%) | 1 | 1 | 0.33 |
3 | 300 | 37 | 7 | 3 | 3 (100%) | 3 | 3 | 1 |