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Table 1 Generation of transgenic plants of hybrid passion fruit KPF4

From: A simple and fast Agrobacterium-mediated transformation system for passion fruit KPF4 (Passiflora edulis f. edulis × Passiflora edulis f. flavicarpa)

Transformation experiment No. of explants infected with Agrobacterium harboring pCAMBIA1301 No. of explants surviving on selection medium No. of explants forming shoot buds on SIM with selection agent No. of shoots developed on SDM with selection agent Rooting on RIM with selection agent No. of transgenic lines with GUS expression No of PCR-positive plant lines Transformation efficiency (%)
1 300 18 5 3 2 (66.67%) 2 2 0.67
2 300 29 6 4 1 (25%) 1 1 0.33
3 300 37 7 3 3 (100%) 3 3 1