Skip to main content
Fig. 3 | Plant Methods

Fig. 3

From: A simple and fast Agrobacterium-mediated transformation system for passion fruit KPF4 (Passiflora edulis f. edulis × Passiflora edulis f. flavicarpa)

Fig. 3

Agrobacterium-mediated transformation of leaf disc explants of passion fruit KPF4 using Agrobacterium strain LBA4404 harboring pCAMBIA1301. a Culture of inoculated leaf disc explants on resting medium; b One-week-old inoculated explants on shoot bud induction medium with selection agent (7.5 mg L−1 hygromycin); c Micro-shoots of leaf explants on SIM containing selection agent (7.5 mg L−1 hygromycin) for 3 weeks; d Elongated shoots after 4 weeks of culture on SDM containing selection agent (7.5 mg L−1 hygromycin); e Rooting test on RIM supplemented with 7.5 mg L−1 hygromycin for 2 weeks

Back to article page