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Fig. 4 | Plant Methods

Fig. 4

From: Rapid, high efficiency virus-mediated mutant complementation and gene silencing in Antirrhinum

Fig. 4

VIGS of the H gene. a Leaves above metamer 4 produce no epidermal hairs from the leaf blade in wild-type plants infected with empty TRV. b VIGS of AmPDS does not cause production of ectopic hairs. c ViGS of H causes ectopic trichomes to form in regions of the leaf blade also showing VIGS of AmPDS. d Not all bleached areas of leaf produce ectopic trichomes (arrowhead). Scale-bars show 5 mm. e Relative H RNA abundance in vegetative apices of plants infected with either TRV:H-AmPDS or TRV:AmPDS. Each bar shows the mean of three biological replicates (each a separate plant or pool of plants) ± its standard error; the p-value is from a Student’s t-test

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