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Fig. 3 | Plant Methods

Fig. 3

From: Rapid, high efficiency virus-mediated mutant complementation and gene silencing in Antirrhinum

Fig. 3

VIGS of the flower asymmetry gene DIV. a About one in five plants infected with virus carrying a fragment of AmPDS alone (middle column) or DIV and AmPDS (right column) showed bleaching of photosynthetic tissues throughout development. b The Antirrhinum corolla consists of two dorsal (D), two lateral (L) and one ventral (V) petal. In uninfected plants (left column) and plants with reduced AmPDS expression (middle), the ventral-most petal (shown with a white bar above it) folds to form the corolla face. c The ventral corolla face bears dense grey-coloured trichomes (T) internally. d The inside of the ventral corolla also has two stripes of yellow trichomes (nectar guides, arrowheads) that extend the full length of the corolla tube in uninfected or TRV:AmPDS infected plants. In TRV:Div-AmPDS infected plants (right column), folding or the ventral-most petal was reduced (b), internal grey trichomes were lost (c) and nectar guides were reduced (d). e Relative Div RNA abundance in young flower buds of plants infected with TRV:Div-AmPDS or TRV:AmPDS. Bars show the mean of three biological replicates (each a separate plant) ± their standard errors; the p value is from a Student’s t-test

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