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Fig. 1 | Plant Methods

Fig. 1

From: Rapid, high efficiency virus-mediated mutant complementation and gene silencing in Antirrhinum

Fig. 1

TRV infection in A. majus. a The T-DNA region of pTRV2sgP, delimited by left and right borders (LB, RB), which expresses the TRV2 RNA (black line) from a double 35S promoter. A GFP ORF was inserted into the multiple cloning site (MCS) 3′ to a sub-genomic promoter (sgP) from Pea early browning virus (PEBV). TRV2sgP encodes coat protein (CP), but lacks 2b and 2c genes, which encode non-structural proteins. TRV1, which encodes the viral replicase, movement protein and 16 K silencing repressor, is not shown. b Representative plants that were either inoculated with virus in which the TRV2 genome was transcribed from empty pTRV2sgP (TRV) or mock-inoculated (mock). c Inoculation with TRV reduced plant growth (shown here for metamer 1–7 stem length, p = 0.025 from a Student’s t-test). d Plants inoculated with virus produced from pTRV2 that carried either a GFP ORF (TRV:GFP) or that did not (TRV). The two images were taken under UV illumination to detect GFP fluorescence. The inoculated leaves are below the ones shown in these two images. e Metamer 2 leaves 12 days after inoculation. The right-hand leaf in each image was rub-inoculated with infectious sap (TRV) or buffer only (mock)

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