Fig. 2

Heat shock induces excision of the reporter gene. a Excision of the GUS gene and expression of NAM-B1 occurs following heat-shock and expression of Cre recombinase. The location of the primers used to validate the GUS excision are shown in black, alongside the expected band size. b Gel image shows the resultant DNA bands before (blue) and after (yellow) 2 h of heat shock at 38 °C was applied to wheat seedlings. This representative figure depicts the results from individual plants from three independent lines, 2020-20-02, 2020-2-1, and 2020-5-1, at the T₁ generation