Fig. 7

Gene cloning of rs1 and rs2. a The genetic map position of the rs1 and rs2. Molecular markers (top) and their physical positions (bottom) were indicated. CEN represent the centromere. b Four candidate genes of rs1 were presented. Numbers indicate the physical position in M bp of candidate genes. Grey arrows indicate genes direction and accession numbers (below). c Schematic of ABCG14 genetic structure. The genomic DNA of ABCG14 contains 5 exons. Black arrows indicate the position of mutation in the exons. d A point mutation (G → A) occurred to rs1 which generated a stop codon in protein-coding sequence is underlined. e A point mutation (G → A) occurred to rs2 and caused a mutation from Glycine to Aspartic acid in amino acid sequence. f Conformation of rs2 as an atabcg14 allele by crossing rs2 with ARR5::eGFP abcg14. Scale bar, 1 cm