Fig. 3
From: A fluorescence-based high-throughput screening method for cytokinin translocation mutants
Screening and confirmation of mutants defective in cytokinin translocation. a Mutant screening with the elevated GFP signal in the roots. M2 seeds were sowed on the square plates (10 cm * 10 cm) and cultured vertically. 6-DAG mutants were observed and screened with the LIS (left). Red arrow indicates the target mutant (right, ×2 magnification). ARR5::eGFP Col-4 and ARR5::eGFP abcg14 were used as the controls. b GFP signal of rs1 to 3 at 5-DAG. White triangles pointing to the tissues with changed GFP signal in roots. DAG, days after germination. Scale bar, 1 mm