Fig. 1

Schematic diagram of the screening system for cytokinin translocation mutants. ARR5::eGFP (RCS2) vector was constructed and ARR5::eGFP transgenic plants were obtained for mutagenesis by EMS. Mutants were screened by GFP signal observation in whole-plate scale under a luminescence imaging system (LIS) and the phenotypes of the mutants were confirmed individually by fluorescence microscope. The exogenous tZ was sprayed into the mutants to further check the mutants defective in shootward long-distance translocation of cytokinins.